Current Protocols in Stem Cell Biology
Table of Contents

Foreword by Zach W. Hall
Preface
Contributors

# Foreword # Preface

Chapter 1 Embryonic and Extraembryonic Stem Cells

• Section A Isolation of Embryonic Stem Cells
  
  • Introduction
    Full Text:
    
  • Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
    
    
  • Unit 1A.2 Derivation of hESC from Intact Blastocysts
    
• Section B Characterization of Embryonic Stem Cells
  
  • Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
    
    
  • Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
    
    
  • Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
    
    
• Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
  
  • Introduction
    Full Text:
    
  • Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
    
    
  • Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
    
    
  • Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
    
  • Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
    
• Section E Extraembryonic Lineages
  
  • Introduction
    Full Text:
    
  • Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte-Like Cells
    
    
  • Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
    
• Section F Mesodermal Lineages
  
  • Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
    
    
  • Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
• Section G Endodermal Lineages
  
  • Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
    
  • Abstract | Full Text:
    
  • Unit 1G.2 Pancreas Differentiation of Mouse ES Cells

• Section A Hematopoietic Stem Cells
  
  • Introduction
    
  • Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density Gradient
    
  • Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
    
    
  • Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood

• 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research

• 2 Standard Laboratory Equipment

Appendix Suppliers

• Selected Suppliers of Reagents and Equipment